CONTACT

Jean-Jacques DIAZ
Group Leader

jean-jacques.diaz
@lyon.unicancer.fr


+33 (0)4 78 78 28 19

Cheney A - 4th floor

Centre Léon Bérard
28 rue Laennec
69373 Lyon Cedex 08
France
DIAZ Jean-Jacques
Chef d'équipe
DR (INSERM)
Téléphone : 04 78 78 28 19
SAURIN Jean-Christophe
PU-PH (HCL)
Téléphone : 04 72 11 03 69
MERTANI Hichem

MCU (UCBL)
hichem.mertani@lyon.unicancer.fr
04 78 78 28 19

CATEZ Frédéric
CR (CNRS)
Téléphone : 04 26 55 68 43
PION Nathalie
Technicienne (CLARA)
Téléphone : 04 26 55 68 43
MARCEL Virginie

CR (Inserm)
virginie.marcel@lyon.unicancer.fr
Téléphone : 04 26 55 68 43

BOUVET Philippe
PRCE (ENS-Lyon)
philippe.bouvet@ens-lyon.fr
Phone : +33 (0)4 69 85 60 91 
MONGELARD Fabien
PRCE (ENS-Lyon)
fabien.mongelard@ens-lyon.fr

Phone : +33 (0)4 69 85 60 91

DALLA VENEZIA Nicole
CR1 (CNRS)
nicole.dalla-venezia@lyon.unicancer.fr

Phone : 04 26 55 67 45

VINCENT Anne
CR1 (CNRS)
anne.vincent@lyon.unicancer.fr

Phone : 04 26 55 67 45

MONIER Karine
IR (CNRS)
karine.monier@lyon.unicancer.fr 

Phone : 04 26 55 67 45

DELAGE Hélène
AI (CNRS)
helene.delage@lyon.unicancer.fr

Phone:  +33 (0)4 69 85 60 93

MARTINEZ Nicole
AT (ENS-Lyon)
Nicole.MARTINEZ@lyon.unicancer.fr

Phone : +33 (0)4 69 85 60 93

GARCIA Maxime
AI (CDD - ANR)
maxime.garcia@lyon.unicancer.fr

Phone: +33 (0)4 26 55 68 43

NGUYEN VAN LONG Flora
PhD student (UCBL - Ligue Nationale Contre le Cancer)
Flora.NGUYENVANLONG@lyon.unicancer.fr

Phone : +33 (0)4 26 55 67 83

LO MONACO Piero
PhD student (UCBL - MESR)
piero.lomonaco@lyon.unicancer.fr

Phone : +33 (0)4 26 55 67 83

CRUZ Elisabeth
PhD Student (ENS - CONACyT)
Elizabeth.CRUZGOMEZ@lyon.unicancer.fr

Phone : 04 26 55 68 43

MALET Lucie
PhD Student (UCBL - Fondation pour la Recherche Médicale)
lucie.malet@lyon.unicancer.fr
BOYER Thomas
Master (EPHE)
thomas.boyer1@estbb.ucly.fr 

Phone : 04 26 55 68 43

TEODORI Laura
Master (University Camerino, Italy - ERASMUS)
laura.teodori@studenti.unicam.it

Phone : 04 26 55 67 83

Voir les objectifs et projets Voir les publications
Collaborations

Ribosome: toward new breast cancer biomarkers?

- Fabrice André, Institut Gustave Roussy, Villejuif, FRANCE
- Jean-Christophe Bourdon, University of Dundee, UK
- David Pérol, Sylvie Chabaud, Isabelle Treilleux, Biostatistics and therapeutic evaluation unit (UBET), Centre Léon Bérard, Lyon, FRANCE
- Yuri Motorin, Virginie Marchand, Lorraine university, Nancy, FRANCE

Role of ribosomes in EMT

- Alain Puisieux, Anne-Pierre Morel, Cancer Cell Plasticity and EMT team, CRCL, Lyon, FRANCE
- Olivier Namy, Paris-Sud XI, Orsay, FRANCE

Role of snRNAs in lung cancer targeted therapy resistance

- Pierre Saintigny, Sandra Cuaran-Ortiz, Translational research laboratory, CLB/CRCL, Lyon, FRANCE
- Alain Viary, the Gilles Thomas Bioinformatics Facility, Centre Léon Bérard, Lyon, FRANCE

Role of rRNA in ribosome activity and translation control

- Olivier Namy, Paris-Sud XI, Orsay, France
- Yuri Motorin, Virginie Marchand, Lorraine university, Nancy, FRANCE
- Marat Yusupov, IGBMC, Strasbourg, FRANCE
- Anne-Catherine Prats, I2MC, Toulouse, FRANCE
- Jérôme Guitton, Lyon Civil Hospitals, Lyon, FRANCE
- Julie Pannequin et Alexandre David, IGF, Montpellier

OBJECTIVES

The Nuclear Domains and Pathologies Team studies the emerging role of ribosomes in cancer development. We identify cancer specific alterations of the ribosomes and of the ribosome production machinery with two aims: first to better understand the process of tumor development and second to apply this knowledge to the development of novel therapeutic and diagnostic strategies for cancer. We focus on models of breast and colon cancer development and on the response to chemotherapies.

PROJECT

Our group has a long time interest on deregulations of ribosome biogenesis that are associated with pathological conditions, and in particular with tumor development and chemo-resistance. Ribosome biogenesis is a fundamental process of cell biology since it produces the ribosomes, the ribonucleoprotein complexes driving all protein synthesis of the cell. Recently, it has emerged that all ribosomes of a cell are not identical, and that the quality of a ribosome can impact on the translation activity, including quality control. 


3D structure of a ribosome from S. Cerevisae at 3, Å resolution. Large subunit : rRNA in blue, proteins in black. Small subunit : rRNA in orange, proteins in grey. © Gabriel Therizols. Rendering using Pymol. Coordinates from Ben-Shem et al. Science 2011. 

> MORE INFORMATION

PUBLICATIONS

2013

Marcel V, Catez F, Diaz JJ. Ribosomes: the future of targeted therapies? Oncotarget. 2013 Oct;4(10):1554-5.

Marcel V, Ghayad SE, Belin S, Therizols G, Morel AP, Solano-Gonzàlez E, Vendrell JA, Hacot S, Mertani HC, Albaret MA, Bourdon JC, Jordan L, Thompson A, Tafer Y, Cong R, Bouvet P, Saurin JC, Catez F, Prats AC, Puisieux A, Diaz JJ. p53 acts as a safeguard of translational control by regulating fibrillarin and rRNA methylation in cancer. Cancer Cell. 2013 Sep 9;24(3):318-30.

Stirbat TV, Mgharbel A, Bodennec S, Ferri K, Mertani HC, Rieu JP, Delanoë-Ayari H. Fine tuning of tissues' viscosity and surface tension through contractility suggests a new role for α-catenin. PLoS One. 2013;8(2):e52554.

Dacheux E, Vincent A, Nazaret N, Combet C, Wierinckx A, Mazoyer S, Diaz JJ, Lachuer J, Venezia ND. BRCA1-Dependent Translational Regulation in Breast Cancer Cells. PLoS One. 2013 Jun 21;8(6):e67313.

2012

Terrier O, Moules V, Carron C, Cartet G, Frobert E, Yver M, Traversier A, Wolff T, Riteau B, Naffakh N, Lina B, Diaz JJ, Rosa-Calatrava M. The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses. Virology. 2012 Oct

Delloye-Bourgeois C, Goldschneider D, Paradisi A, Therizols G, Belin S, Hacot S, Rosa-Calatrava M, Scoazec JY, Diaz JJ, Bernet A, Mehlen P. Nucleolar localization of a netrin-1 isoform enhances tumor cell proliferation. Sci Signal. 2012 Aug 7;5(236):ra57.

Greco A, Arata L, Soler E, Gaume X, Couté Y, Hacot S, Callé A, Monier K, Epstein AL, Sanchez JC, Bouvet P, Diaz JJ. Nucleolin interacts with US11 protein of herpes simplex virus 1 and is involved in its trafficking. J Virol. 2012

2011

Nguyen-Lefebvre AT, Gonin-Giraud S, Scherl A, Arboit P, Granger L, Sanchez JC, Diaz JJ, Gandrillon O, Madjar JJ. Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. J Proteomics. 2011 Feb 1;74(2):167-85.

Terrier O, Josset L, Textoris J, Marcel V, Cartet G, Ferraris O, N'Guyen C, Lina B, Diaz JJ, Bourdon JC and Rosa-Calatrava M. Cellular transcriptional profiling in human lung epithelial cells infected by different subtypes of influenza A viruses reveals an overall down-regulation of the host p53 pathway. Virol. J. 2011 Jun 8;8(1):285.

Chiesa J, Ferrer C, Arnould C, Vouyovitch CM, Diaz JJ, Gonzalez S, Mares P, Morel G, Wu Z-S, Zhu T, Lobie PE. and Mertani HC.Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells. J Clin Endoc Metab 2011, 96(9):

Auclair J, Vaissière T, Desseigne F, Lasset C, Bonadona V, Giraud S, Saurin JC, Joly MO, Leroux D, Faivre L, Audoynaud C, Montmain G, Ruano E, Herceg Z, Puisieux A, Wang Q. Intensity-dependent constitutional MLH1 promoter methylation leads to early onset of colorectal cancer by affecting both alleles. Genes Chromosomes Cancer. 2011 Mar;50(3):178-85.

El Fajoui, Z., Toscano, F., Jacquemin, G., Abello, J., Scoazec, J.Y., Micheau, O., Saurin, J.C. Oxaliplatin Sensitizes Human Colon Cancer Cells to TRAIL through JNK-Dependent Phosphorylation of BclxL, Gastroenterology, 141, 663-673, (2011).

2010

Hacot S, Coute Y, Belin S, Albaret MA, Mertani HC, Sanchez JC, Rosa-Calatrava M, Diaz JJ. Isolation of nucleoli. Curr Protoc Cell Biol.2010 Jun;Chapter 3:Unit3.36.

Belin, S., Kindbeiter, K., Hacot, S., Albaret, MA., Roca-Martinez, JX., Thérizols, G., Grosso., O. & Diaz., J-J. Uncoupling ribosome biogenesis regulation from RNA polymerase I activity during herpes simplex type 1 infection. RNA, 16, 131-140 (2010).

Josset L, Textoris J, Loriod B, Ferraris O, Moules V, Lina B, N'Guyen C, Diaz JJ, Rosa-Calatrava M. Gene Expression Signature-Based Screening Identifies New Broadly Effective Influenza A. PLoS ONE 5(10): e13169 (2010).

Belin S, Hacot S, Daudignon L, Therizols G, Pourpe S, Mertani HC, Rosa-Calatrava M, Diaz JJ. Purification of ribosomes from human cell lines. Curr Protoc Cell Biol. 2010 Dec;Chapter 3:Unit 3.40.

2009

Juban, G., Giraud, G., Guyot, B., Belin, S., Diaz, J-J., Starck, J., Guillouf, C., Moreau-Gachelin, F. & Morlé, F. Spi-1 and Fli-1 directly activate common target genes involved in ribosome biogenesis in Friend erythroleukemic cells. Mol Cell Biol, 29, 2852-64 (2009).

Belin, S., Beghin, A., Solano-Gonzàlez, E., Laurent Bezin, L., Brunet-Manquat, S., Textoris, J., Prats, A-C., Mertani, H.M., Charles Dumontet, C. & Diaz, J-J. Dysregulation of Ribosome Biogenesis and Translational Capacity Is Associated with Tumor Progression of Human Breast Cancer Cells. PLoS ONE 4(9): e7147 (2009).

Beghin, A., Belin, S., Sleiman, RH., Brunet-Manquat, S., Goddard, S., Tabone, E., Treilleux, I., Poupon, MF., Diaz, J-J. & Charles Dumontet.ADP Ribosylation Factor Like 2 (Arl2) Regulates Breast Tumor Aggressivity in Immunodeficient Mice. PLoS ONE, 2009, 4(10): e7478.

2008

Coute, Y., Kindbeiter, K., Belin, S., Dieckmann, R., Duret, L., Bezin, L., Sanchez, J. C. & Diaz, J. J. ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in Ribosome biogenesis. Mol Cell Proteomics, 7, 546-59 (2008).

Callé, A., Ugrinova, I., Epstein, A.L., Bouvet, P., Diaz, J-J. & Greco, A. Nucleolin is required for an efficient herpes simplex virus type 1 infection. J. Virol. 82, 4762-73 (2008).

Diaz, J-J., Couderc, C., Couté, Y., Poncet, G., Pourpe, S., Hacot, S., Borson-Chazot, F., Aguerra, K., Scoazec, JY., Sanchez, JC. & Roche, C. A proteomic approach of the endocrine tumors. Annales d’Endocrinologie, 69, 138-142 (2008)

2006

Couté, Y., Burgess, J.A., Diaz, J-J., Chichester, C., Lisacek, F., Greco, A. & Sanchez, J-C. Deciphering the human nucleolar proteome. Mass Spectrom Rev. 25, 215-34 (2006).)

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